2025-09-08 Time Series RNA-seq Plate Prep

Prep and submit RNA for TimeSeries Project, September 8-9, 2025 (to be sent to Genohub Sequencer in OK)

Loaded 500 ng of RNA into plate, did not dilute all samples to the exact same concentration but just sent appropriate volume to send 500ng. If a sample was very highly concentrated (volume required for 500 ng was less than 20 uL), I diluted it 2X to increase the volume being sent.

Samples

All Pocillopora acuta, Porites compressa, and Montipora capitata – 126 samples from 7 timepoints, 6 samples per species per timepoint.

Extraction Order	fragment_name	Species	Extraction Date	RNA_Extraction_Status	RNA_QBIT_AVG	Amount RNA loaded (500 ng)	Amount of water	Final_vol	Final_ng	Final_Conc	Plate	Well	Plate_Well	Plate well order
87	POC_R0_C3	Pocillopora_acuta	7/31/25	complete	23.3	21	0	21	500	23.3	Plate1	A1	Plate1_A1	1
9	POR_R12_H1	Porites_compressa	7/9/25	complete	15.7	32	0	32	500	15.7	Plate1	B1	Plate1_B1	2
55	MON_R72_H1	Montipora_capitata	7/23/25	complete	19.8	25	0	25	500	19.8	Plate1	C1	Plate1_C1	3
13	MON_R72_H2	Montipora_capitata	7/14/25	complete	13.5	37	0	37	500	13.5	Plate1	D1	Plate1_D1	4
20	POR_R72_C1	Porites_compressa	7/15/25	complete	11.9	42	0	42	500	11.9	Plate1	E1	Plate1_E1	5
57	POR_R0_H2	Porites_compressa	7/23/25	complete	24	21	0	21	500	24	Plate1	F1	Plate1_F1	6
50	MON_R120_H1	Montipora_capitata	7/22/25	complete	15.9	31	0	31	500	15.9	Plate1	G1	Plate1_G1	7
69	POC_R72_C2	Pocillopora_acuta	7/28/25	complete	16.6	30	0	30	500	16.6	Plate1	H1	Plate1_H1	8
29	POC_R24_C2	Pocillopora_acuta	7/16/25	complete	29.3	17	17	34	500	14.65	Plate1	A2	Plate1_A2	9
11	POR_R72_H3	Porites_compressa	7/9/25	complete	23.2	22	0	22	500	23.2	Plate1	B2	Plate1_B2	10
52	MON_R12_H2	Montipora_capitata	7/23/25	complete	10.2	49	0	49	500	10.2	Plate1	C2	Plate1_C2	11
22	POR_R1_C3	Porites_compressa	7/15/25	complete	20.1	25	0	25	500	20.1	Plate1	D2	Plate1_D2	12
111	MON_R1_H1	Montipora_capitata	9/8/25	complete	13.45	37	0	37	500	13.45	Plate1	E2	Plate1_E2	13
39	POC_R12_H2	Pocillopora_acuta	7/21/25	complete	11.8	42	0	42	500	11.8	Plate1	F2	Plate1_F2	14
42	POR_R24_C2	Porites_compressa	7/21/25	complete	17.6	28	0	28	500	17.6	Plate1	G2	Plate1_G2	15
124	MON_R12_C3	Montipora_capitata	9/5/25	complete	33.1	15	15	30	500	16.55	Plate1	H2	Plate1_H2	16
86	POC_R120_H3	Pocillopora_acuta	7/31/25	complete	22	23	0	23	500	22	Plate1	A3	Plate1_A3	17
94	POC_R12_C1	Pocillopora_acuta	8/4/25	complete	24.2	21	0	21	500	24.2	Plate1	B3	Plate1_B3	18
54	POC_R120_C1	Pocillopora_acuta	7/23/25	complete	19.6	26	0	26	500	19.6	Plate1	C3	Plate1_C3	19
107	POR_R24_H2	Porites_compressa	8/7/25	complete	29.3	17	17	34	500	14.65	Plate1	D3	Plate1_D3	20
114	MON_R3_H1	Montipora_capitata	9/8/25	complete	15.35	33	0	33	500	15.35	Plate1	E3	Plate1_E3	21
122	MON_R72_C1	Montipora_capitata	9/5/25	complete	24.3	21	0	21	500	24.3	Plate1	F3	Plate1_F3	22
85	POR_R0_H1	Porites_compressa	7/31/25	complete	20.5	24	0	24	500	20.5	Plate1	G3	Plate1_G3	23
121	MON_R1_C3	Montipora_capitata	9/5/25	complete	15.6	32	0	32	500	15.6	Plate1	H3	Plate1_H3	24
108	MON_R24_C2	Montipora_capitata	8/29/25	complete	10.4	48	0	48	500	10.4	Plate1	A4	Plate1_A4	25
95	MON_R12_C2	Montipora_capitata	8/5/25	complete	13.7	36	0	36	500	13.7	Plate1	B4	Plate1_B4	26
83	MON_R0_H3	Montipora_capitata	7/31/25	complete	12.2	41	0	41	500	12.2	Plate1	C4	Plate1_C4	27
28	POC_R3_C2	Pocillopora_acuta	7/16/25	complete	26.5	19	19	38	500	13.25	Plate1	D4	Plate1_D4	28
92	POC_R1_C1	Pocillopora_acuta	8/4/25	complete	19.5	26	0	26	500	19.5	Plate1	E4	Plate1_E4	29
72	POR_R3_C1	Porites_compressa	7/28/25	complete	21.8	23	0	23	500	21.8	Plate1	F4	Plate1_F4	30
119	MON_R3_C3	Montipora_capitata	9/5/25	complete	14.3	35	0	35	500	14.3	Plate1	G4	Plate1_G4	31
7	POR_R0_C2	Porites_compressa	7/9/25	complete	12.8	39	0	39	500	12.8	Plate1	H4	Plate1_H4	32
93	POR_R3_H3	Porites_compressa	8/4/25	complete	20	25	0	25	500	20	Plate1	A5	Plate1_A5	33
68	MON_R72_C3	Montipora_capitata	7/28/25	complete	13.2	38	0	38	500	13.2	Plate1	B5	Plate1_B5	34
118	MON_R24_C3	Montipora_capitata	9/5/25	complete	13.6	37	0	37	500	13.6	Plate1	C5	Plate1_C5	35
120	MON_R12_H1	Montipora_capitata	9/5/25	complete	14.3	35	0	35	500	14.3	Plate1	D5	Plate1_D5	36
14	POC_R0_C2	Pocillopora_acuta	7/14/25	complete	18.1	28	0	28	500	18.1	Plate1	E5	Plate1_E5	37
61	MON_R1_H2	Montipora_capitata	7/24/25	complete	12.8	39	0	39	500	12.8	Plate1	F5	Plate1_F5	38
104	POC_R72_C3	Pocillopora_acuta	8/6/25	complete	22.4	22	0	22	500	22.4	Plate1	G5	Plate1_G5	39
84	POR_R24_C3	Porites_compressa	7/31/25	complete	13.9	36	0	36	500	13.9	Plate1	H5	Plate1_H5	40
67	MON_R1_C1	Montipora_capitata	7/28/25	complete	12.8	39	0	39	500	12.8	Plate1	A6	Plate1_A6	41
71	POR_R12_H3	Porites_compressa	7/28/25	complete	21	24	0	24	500	21	Plate1	B6	Plate1_B6	42
49	MON_R12_H3	Montipora_capitata	7/22/25	complete	15.8	32	0	32	500	15.8	Plate1	C6	Plate1_C6	43
40	MON_R72_H3	Montipora_capitata	7/21/25	complete	13	38	0	38	500	13	Plate1	D6	Plate1_D6	44
65	POC_R1_H1	Pocillopora_acuta	7/24/25	complete	24.6	20	0	20	500	24.6	Plate1	E6	Plate1_E6	45
62	POR_R120_H2	Porites_compressa	7/24/25	complete	19.2	26	0	26	500	19.2	Plate1	F6	Plate1_F6	46
3	POR_R120_H1	Porites_compressa	7/8/25	complete	17.8	28	0	28	500	17.8	Plate1	G6	Plate1_G6	47
88	POC_R72_H1	Pocillopora_acuta	7/31/25	complete	28.9	17	17	35	500	14.45	Plate1	H6	Plate1_H6	48
21	POC_R1_C3	Pocillopora_acuta	7/15/25	complete	13.6	37	0	37	500	13.6	Plate1	A7	Plate1_A7	49
77	MON_R24_C1	Montipora_capitata	7/29/25	complete	22.9	22	0	22	500	22.9	Plate1	B7	Plate1_B7	50
24	POR_R72_H2	Porites_compressa	7/16/25	complete	10.1	50	0	50	500	10.1	Plate1	C7	Plate1_C7	51
105	POC_R0_H2	Pocillopora_acuta	8/7/25	complete	10	50	0	50	500	10	Plate1	D7	Plate1_D7	52
23	POC_R24_H1	Pocillopora_acuta	7/15/25	complete	25.8	19	19	39	500	12.9	Plate1	E7	Plate1_E7	53
66	POR_R0_C3	Porites_compressa	7/24/25	complete	24.9	20	0	20	500	24.9	Plate1	F7	Plate1_F7	54
25	POR_R72_C3	Porites_compressa	7/16/25	complete	16.6	30	0	30	500	16.6	Plate1	G7	Plate1_G7	55
35	POC_R24_H3	Pocillopora_acuta	7/17/25	complete	14.9	34	0	34	500	14.9	Plate1	H7	Plate1_H7	56
106	POC_R3_H2	Pocillopora_acuta	8/7/25	complete	17.3	29	0	29	500	17.3	Plate1	A8	Plate1_A8	57
34	POR_R3_C3	Porites_compressa	7/17/25	complete	14.8	34	0	34	500	14.8	Plate1	B8	Plate1_B8	58
96	POC_R3_H3	Pocillopora_acuta	8/5/25	complete	13.9	36	0	36	500	13.9	Plate1	C8	Plate1_C8	59
103	POR_R0_H3	Porites_compressa	8/6/25	complete	22.1	23	0	23	500	22.1	Plate1	D8	Plate1_D8	60
15	POR_R3_C2	Porites_compressa	7/14/25	complete	19	26	0	26	500	19	Plate1	E8	Plate1_E8	61
33	POR_R1_H2	Porites_compressa	7/17/25	complete	14.7	34	0	34	500	14.7	Plate1	F8	Plate1_F8	62
81	POC_R24_C3	Pocillopora_acuta	7/29/25	complete	28.7	17	17	35	500	14.35	Plate1	G8	Plate1_G8	63
101	POC_R0_H3	Pocillopora_acuta	8/6/25	complete	11.4	44	0	44	500	11.4	Plate1	H8	Plate1_H8	64
18	POR_R24_C1	Porites_compressa	7/14/25	complete	33.7	15	15	30	500	16.85	Plate1	A9	Plate1_A9	65
8	POR_R1_C1	Porites_compressa	7/9/25	complete	15.2	33	0	33	500	15.2	Plate1	B9	Plate1_B9	66
74	MON_R1_C2	Montipora_capitata	7/29/25	complete	10.2	49	0	49	500	10.2	Plate1	C9	Plate1_C9	67
90	MON_R72_C2	Montipora_capitata	8/4/25	complete	11.8	42	0	42	500	11.8	Plate1	D9	Plate1_D9	68
110	MON_R24_H1	Montipora_capitata	8/29/25	complete	14.8	34	0	34	500	14.8	Plate1	E9	Plate1_E9	69
56	POC_R1_H2	Pocillopora_acuta	7/23/25	complete	22.7	22	0	22	500	22.7	Plate1	F9	Plate1_F9	70
12	POR_R72_C2	Porites_compressa	7/9/25	complete	25.8	19	19	39	500	12.9	Plate1	G9	Plate1_G9	71
125	MON_R120_C1	Montipora_capitata	9/5/25	complete	36.55	14	14	27	500	18.275	Plate1	H9	Plate1_H9	72
6	POR_R1_H1	Porites_compressa	7/9/25	complete	10.4	48	0	48	500	10.4	Plate1	A10	Plate1_A10	73
43	POC_R3_C3	Pocillopora_acuta	7/21/25	complete	17.7	28	0	28	500	17.7	Plate1	B10	Plate1_B10	74
99	POC_R72_H2	Pocillopora_acuta	8/5/25	complete	26.1	19	19	38	500	13.05	Plate1	C10	Plate1_C10	75
115	MON_R3_C1	Montipora_capitata	9/5/25	complete	12	42	0	42	500	12	Plate1	D10	Plate1_D10	76
27	POR_R0_C1	Porites_compressa	7/16/25	complete	24	21	0	21	500	24	Plate1	E10	Plate1_E10	77
59	POC_R24_H2	Pocillopora_acuta	7/23/25	complete	30.1	17	17	33	500	15.05	Plate1	F10	Plate1_F10	78
113	MON_R24_H3	Montipora_capitata	9/9/25	complete	9.215	54	0	54	500	9.215	Plate1	G10	Plate1_G10	79
2	POR_R120_C3	Porites_compressa	7/8/25	complete	15.8	32	0	32	500	15.8	Plate1	H10	Plate1_H10	80
63	POC_R24_C1	Pocillopora_acuta	7/24/25	complete	21	24	0	24	500	21	Plate1	A11	Plate1_A11	81
4	POR_R3_H2	Porites_compressa	7/8/25	complete	21	24	0	24	500	21	Plate1	B11	Plate1_B11	82
26	POC_R12_C2	Pocillopora_acuta	7/16/25	complete	21.2	24	0	24	500	21.2	Plate1	C11	Plate1_C11	83
116	MON_R120_C3	Montipora_capitata	9/5/25	complete	13.1	38	0	38	500	13.1	Plate1	D11	Plate1_D11	84
70	POC_R12_H1	Pocillopora_acuta	7/28/25	complete	20	25	0	25	500	20	Plate1	E11	Plate1_E11	85
19	POR_R1_H3	Porites_compressa	7/15/25	complete	11.8	42	0	42	500	11.8	Plate1	F11	Plate1_F11	86
98	POR_R120_C2	Porites_compressa	8/5/25	complete	20.8	24	0	24	500	20.8	Plate1	G11	Plate1_G11	87
97	POR_R12_H2	Porites_compressa	8/5/25	complete	18.5	27	0	27	500	18.5	Plate1	H11	Plate1_H11	88
112	MON_R0_H1	Montipora_capitata	9/8/25	complete	8	30	0	30	240	8	Plate1	A12	Plate1_A12	89
126	MON_R120_H2	Montipora_capitata	9/5/25	complete	37.75	13	13	26	500	18.875	Plate1	B12	Plate1_B12	90
37	POC_R72_H3	Pocillopora_acuta	7/17/25	complete	23.3	21	0	21	500	23.3	Plate1	C12	Plate1_C12	91
51	POC_R120_H2	Pocillopora_acuta	7/22/25	complete	17.6	28	0	28	500	17.6	Plate1	D12	Plate1_D12	92
45	POC_R1_H3	Pocillopora_acuta	7/22/25	complete	13.9	36	0	36	500	13.9	Plate1	E12	Plate1_E12	93
82	MON_R3_H2	Montipora_capitata	7/31/25	complete	10	50	0	50	500	10	Plate1	F12	Plate1_F12	94
10	POR_R24_H3	Porites_compressa	7/9/25	complete	16.8	30	0	30	500	16.8	Plate1	G12	Plate1_G12	95
38	MON_R0_C1	Montipora_capitata	7/21/25	complete	10.2	49	0	49	500	10.2	Plate1	H12	Plate1_H12	96
123	MON_R3_H3	Montipora_capitata	9/5/25	complete	26.9	19	19	37	500	13.45	Plate2	A1	Plate2_A1	97
117	MON_R24_H2	Montipora_capitata	9/5/25	complete	13.3	38	0	38	500	13.3	Plate2	B1	Plate2_B1	98
89	MON_R3_C2	Montipora_capitata	8/4/25	complete	10.2	49	0	49	500	10.2	Plate2	C1	Plate2_C1	99
1	POR_R72_H1	Porites_compressa	7/8/25	complete	10.7	47	0	47	500	10.7	Plate2	D1	Plate2_D1	100
53	MON_R1_H3	Montipora_capitata	7/23/25	complete	11	45	0	45	500	11	Plate2	E1	Plate2_E1	101
79	POC_R0_H1	Pocillopora_acuta	7/29/25	complete	23.9	21	0	21	500	23.9	Plate2	F1	Plate2_F1	102
17	POC_R12_C3	Pocillopora_acuta	7/14/25	complete	28.6	17	17	35	500	14.3	Plate2	G1	Plate2_G1	103
100	POC_R0_C1	Pocillopora_acuta	8/5/25	complete	26.3	19	19	38	500	13.15	Plate2	H1	Plate2_H1	104
44	POR_R12_C2	Porites_compressa	7/21/25	complete	22.7	22	0	22	500	22.7	Plate2	A2	Plate2_A2	105
47	POC_R3_H1	Pocillopora_acuta	7/22/25	complete	14.3	35	0	35	500	14.3	Plate2	B2	Plate2_B2	106
30	MON_R0_C3	Montipora_capitata	7/17/25	complete	10.4	48	0	48	500	10.4	Plate2	C2	Plate2_C2	107
58	POR_R3_H1	Porites_compressa	7/23/25	complete	27.5	18	18	36	500	13.75	Plate2	D2	Plate2_D2	108
80	POR_R12_C1	Porites_compressa	7/29/25	complete	24.7	20	0	20	500	24.7	Plate2	E2	Plate2_E2	109
75	MON_R0_H2	Montipora_capitata	7/29/25	complete	11.2	45	0	45	500	11.2	Plate2	F2	Plate2_F2	110
64	POC_R120_C2	Pocillopora_acuta	7/24/25	complete	22.4	22	0	22	500	22.4	Plate2	G2	Plate2_G2	111
31	POC_R72_C1	Pocillopora_acuta	7/17/25	complete	13	38	0	38	500	13	Plate2	H2	Plate2_H2	112
60	MON_R120_H3	Montipora_capitata	7/23/25	complete	30.9	16	16	32	500	15.45	Plate2	A3	Plate2_A3	113
91	POR_R12_C3	Porites_compressa	8/4/25	complete	18.5	27	0	27	500	18.5	Plate2	B3	Plate2_B3	114
109	MON_R120_C2	Montipora_capitata	8/29/25	complete	12.9	39	0	39	500	12.9	Plate2	C3	Plate2_C3	115
102	POR_R1_C2	Porites_compressa	8/6/25	complete	18	28	0	28	500	18	Plate2	D3	Plate2_D3	116
73	POC_R1_C2	Pocillopora_acuta	7/28/25	complete	25.4	20	0	20	500	25.4	Plate2	E3	Plate2_E3	117
48	MON_R0_C2	Montipora_capitata	7/22/25	complete	15.4	32	0	32	500	15.4	Plate2	F3	Plate2_F3	118
5	POR_R24_H1	Porites_compressa	7/9/25	complete	10	50	0	50	500	10	Plate2	G3	Plate2_G3	119
41	POC_R120_C3	Pocillopora_acuta	7/21/25	complete	14.7	34	0	34	500	14.7	Plate2	H3	Plate2_H3	120
32	MON_R12_C1	Montipora_capitata	7/17/25	complete	13.5	37	0	37	500	13.5	Plate2	A4	Plate2_A4	121
76	POC_R120_H1	Pocillopora_acuta	7/29/25	complete	16.4	30	0	30	500	16.4	Plate2	B4	Plate2_B4	122
78	POR_R120_H3	Porites_compressa	7/29/25	complete	23.4	21	0	21	500	23.4	Plate2	C4	Plate2_C4	123
46	POC_R12_H3	Pocillopora_acuta	7/22/25	complete	13.9	36	0	36	500	13.9	Plate2	D4	Plate2_D4	124
16	POC_R3_C1	Pocillopora_acuta	7/14/25	complete	20.7	24	0	24	500	20.7	Plate2	E4	Plate2_E4	125
36	POR_R120_C1	Porites_compressa	7/17/25	complete	16.7	30	0	30	500	16.7	Plate2	F4	Plate2_F4	126

Prepped plates were covered with foil and placed in -80 until shipping:

Written on September 8, 2025