2024
May 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • May 14, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • May 13, 2024
April 2024
- Using renv for Reproducible Environments in R • April 30, 2024
- Fixing Coral Tissues for LCM • April 29, 2024
March 2024
- TagSeq Gene Expression Pipeline • March 12, 2024
February 2024
- Extracting RNA and DNA from Fixed P. acuta Sections (newly fixed 2/8/24) • February 15, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned P. comp and M. cap • February 9, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned P. comp and M. cap • February 1, 2024
January 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Genomic DNA Purification Kit • January 31, 2024
- Sending FF and PX corals for snSeq and Spatial Trials • January 29, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Qiagen AllPrep DNA/RNA FFPE Kit Protocol • January 25, 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Total RNA/MicroRNA Purification Kit • January 25, 2024
2023
</ul>October 2023
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
August 2023
- Protocol for Cryosectioning Tissues (for FISH, Immunofluorescence, and LCM) • August 31, 2023
- Fixing Coral Tissues for Protocol Testing • August 30, 2023
- Protocol for Cryoembedding Tissues in OCT Media for Cryosectioning (for FISH, Immunofluorescence, and LCM) • August 22, 2023
- Protocol for Fixing Coral Tissues with PAXgene Tissue Fixative followed by EDTA Decalcification • August 21, 2023
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
June 2023
- Putnam Lab Physiology Surface Area by Wax Dipping • June 30, 2023
- Putnam Lab Physiology Ash Free Dry Weight Protocol • June 23, 2023
- Putnam Lab Physiology Soluble Protein Protocol • June 22, 2023
- Putnam Lab Physiology Symbiont Density Protocol • June 19, 2023
- Putnam Lab Physiology Chlorophyll Protocol • June 19, 2023
- Putnam Lab Physiology Airbrushing Protocol • June 19, 2023
May 2023
- 2023-05-09 Project Summary, 2022-2023 RNA and DNA Extractions for ENCORE MDEC Project • May 9, 2023
- 2023-05-08 ENCORE DLAB, MCAV, and MDEC TagSeq (RNA) Plate Prep • May 8, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
April 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-13 Paxgene Fixation, Stabilization, and Extraction Test • April 13, 2023
- 2023-04-11 E5 Sites 2 & 3 Species ID (POC and POR) via PCR and Sanger Sequencing • April 11, 2023
- 2023-04-11 Heron Island Pdam Species ID (mtORF confirmation) • April 11, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
March 2023
- 2023-03-21 Paxgene Fixation, Stabilization, and Extraction Test • March 21, 2023
- 2023-03-15 E5 Site 1 Species ID (POC and POR) via PCR and Sanger Sequencing • March 15, 2023
- PCR Protocol • March 14, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
February 2023
- Point Judith Oyster RNAseq Analysis • February 27, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
January 2023
- 2023-01-27 Project Summary Barott and Brown Pdam RNA and DNA Extractions • January 27, 2023
- 2023-01-23 E5 ACR Deep Dive RNA and DNA Extractions • January 23, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- Point Judith Oyster RNAseq QC • January 12, 2023
2022
</ul>December 2022
- 2022-12-02 Barott and Brown Pdam RNA Plate Prep • December 2, 2022
- 2022-12-02 Barott and Brown Pdam RNA Nanodrop • December 2, 2022
November 2022
- 2022-11-18 Barott and Brown Pdam RNA and DNA Extractions • November 18, 2022
- 2022-11-11 Barott and Brown Pdam RNA and DNA Extractions • November 11, 2022
- 2022-11-08 Barott and Brown Pdam RNA and DNA Extractions • November 8, 2022
- 2022-11-02 Barott and Brown Pdam RNA and DNA Extractions • November 2, 2022
October 2022
- 2022-10-31 ENCORE RNA and DNA Extractions • October 31, 2022
- 2022-10-27 Barott and Brown Pdam RNA and DNA Extractions • October 27, 2022
- 2022-10-26 ENCORE RNA and DNA Extractions • October 26, 2022
- 2022-10-24 ENCORE RNA and DNA Extractions • October 24, 2022
- 2022-10-21 ENCORE RNA and DNA Extractions • October 21, 2022
- 2022-10-19 ENCORE RNA and DNA Extractions • October 19, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- Adult Coral RNA and DNA Extraction using the Zymo Quick-DNA/RNA™ Miniprep Plus Kit • October 3, 2022
- PPP Lab RNA Tape Station Protocol • October 2, 2022
- dsDNA and RNA Qubit Quantification Protocol • October 2, 2022
- RNA Nanodrop Purity Check and Quantification Protocol • October 2, 2022
- PPP Lab Agarose Gel Protocol • October 2, 2022