Acropora(1) •
Bioinformatics(1) •
Coding(1) •
DNA(60) •
Diploria labyrinthiformis(14) •
E5(4) •
ENCORE(20) •
Fixative(16) •
LCM(34) •
LCM Pilot(3) •
Library Prep(8) •
Madracis decactis(15) •
Montastraea cavernosa(14) •
Montipora(2) •
OysterPaper(2) •
PAXgene(19) •
Physiology(6) •
Pocillopora(43) •
Pocillopora damicornis(9) •
Porites(14) •
Porites astreoides(18) •
Protocol(20) •
RNA(58) •
Seriatopora(1) •
TagSeq(1) •
snSeq(1)
Acropora 1
- 2023-01-23 E5 ACR Deep Dive RNA and DNA Extractions • January 23, 2023
Bioinformatics 1
- Gene Expression Pipeline • March 12, 2024
Coding 1
- Using renv for Reproducible Environments in R • April 30, 2024
DNA 60
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2022-10-19 ENCORE RNA and DNA Extractions • October 19, 2022
- 2022-10-21 ENCORE RNA and DNA Extractions • October 21, 2022
- 2022-10-24 ENCORE RNA and DNA Extractions • October 24, 2022
- 2022-10-26 ENCORE RNA and DNA Extractions • October 26, 2022
- 2022-10-27 Barott and Brown Pdam RNA and DNA Extractions • October 27, 2022
- 2022-10-31 ENCORE RNA and DNA Extractions • October 31, 2022
- 2022-11-02 Barott and Brown Pdam RNA and DNA Extractions • November 2, 2022
- 2022-11-08 Barott and Brown Pdam RNA and DNA Extractions • November 8, 2022
- 2022-11-11 Barott and Brown Pdam RNA and DNA Extractions • November 11, 2022
- 2022-11-18 Barott and Brown Pdam RNA and DNA Extractions • November 18, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-01-23 E5 ACR Deep Dive RNA and DNA Extractions • January 23, 2023
- 2023-01-27 Project Summary Barott and Brown Pdam RNA and DNA Extractions • January 27, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-03-15 E5 Site 1 Species ID (POC and POR) via PCR and Sanger Sequencing • March 15, 2023
- 2023-03-21 Paxgene Fixation, Stabilization, and Extraction Test • March 21, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-11 E5 Sites 2 & 3 Species ID (POC and POR) via PCR and Sanger Sequencing • April 11, 2023
- 2023-04-11 Heron Island Pdam Species ID (mtORF confirmation) • April 11, 2023
- 2023-04-13 Paxgene Fixation, Stabilization, and Extraction Test • April 13, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
- 2023-05-09 Project Summary, 2022-2023 RNA and DNA Extractions for ENCORE MDEC Project • May 9, 2023
- 2024-08-01 E5 Time Series RNA and DNA Re-Extractions • August 1, 2024
- 2024-09-08 LCM Experiment Test Extraction • September 8, 2024
- 2024-09-13 LCM Experiment Extractions • September 13, 2024
- Adult Coral RNA and DNA Extraction using the Zymo Quick-DNA/RNA™ Miniprep Plus Kit • October 3, 2022
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Genomic DNA Purification Kit • January 31, 2024
- Continued Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 30, 2024
- Fixing Coral Tissues for Protocol Testing • August 30, 2023
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Further Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 24, 2024
- LCM Pico Methyl-Seq Library Prep • September 17, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 1 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 2 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 3 • December 15, 2024
- LCM Zymo Trio WGBS Library Prep • December 3, 2024
- PCR Protocol • March 14, 2023
- PPP Lab Agarose Gel Protocol • October 2, 2022
- Qiagen AllPrep DNA/RNA FFPE Kit Protocol • January 25, 2024
- Suucessful? Testing of Charm Biotech DNA Extraction on LCM-d P. acuta • June 26, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • June 17, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • May 14, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
- Testing Pico Methyl-Seq Library Prep • August 27, 2024
- Testing Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 19, 2024
- Testing Zymo cfDNA DNA Extraction on Cryosectioned and LCM-d P. acuta • May 28, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa • July 19, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa and P. acuta • July 24, 2024
- dsDNA and RNA Qubit Quantification Protocol • October 2, 2022
Diploria labyrinthiformis 14
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
- 2023-05-08 ENCORE DLAB, MCAV, and MDEC TagSeq (RNA) Plate Prep • May 8, 2023
E5 4
- 2023-01-23 E5 ACR Deep Dive RNA and DNA Extractions • January 23, 2023
- 2023-03-15 E5 Site 1 Species ID (POC and POR) via PCR and Sanger Sequencing • March 15, 2023
- 2023-04-11 E5 Sites 2 & 3 Species ID (POC and POR) via PCR and Sanger Sequencing • April 11, 2023
- 2024-08-01 E5 Time Series RNA and DNA Re-Extractions • August 1, 2024
ENCORE 20
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2022-10-19 ENCORE RNA and DNA Extractions • October 19, 2022
- 2022-10-21 ENCORE RNA and DNA Extractions • October 21, 2022
- 2022-10-24 ENCORE RNA and DNA Extractions • October 24, 2022
- 2022-10-26 ENCORE RNA and DNA Extractions • October 26, 2022
- 2022-10-31 ENCORE RNA and DNA Extractions • October 31, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
- 2023-05-08 ENCORE DLAB, MCAV, and MDEC TagSeq (RNA) Plate Prep • May 8, 2023
- 2023-05-09 Project Summary, 2022-2023 RNA and DNA Extractions for ENCORE MDEC Project • May 9, 2023
Fixative 16
- 2023-03-21 Paxgene Fixation, Stabilization, and Extraction Test • March 21, 2023
- 2023-04-13 Paxgene Fixation, Stabilization, and Extraction Test • April 13, 2023
- 2024-08-28 LCM Experiment Sample Prep • August 28, 2024
- Extracting RNA and DNA from Fixed P. acuta Sections (newly fixed 2/8/24) • February 15, 2024
- Fixing Coral Tissues for LCM • April 29, 2024
- Fixing Coral Tissues for Protocol Testing • August 30, 2023
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Fixing POC and POR Coral Tissues for LCM • July 3, 2024
- Protocol for Cryoembedding Tissues in OCT Media for Cryosectioning (for FISH, Immunofluorescence, and LCM) • August 22, 2023
- Protocol for Cryosectioning Tissues (for FISH, Immunofluorescence, and LCM) • August 31, 2023
- Protocol for Fixing Coral Tissues with PAXgene Tissue Fixative followed by EDTA Decalcification • August 21, 2023
- Testing Charm Biotech DNA Extraction on Cryosectioned P. comp and M. cap • February 9, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned P. comp and M. cap • February 1, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
LCM 34
- 2024-08-28 LCM Experiment Sample Prep • August 28, 2024
- 2024-09-08 LCM Experiment Test Extraction • September 8, 2024
- 2024-09-13 LCM Experiment Extractions • September 13, 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Genomic DNA Purification Kit • January 31, 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Total RNA/MicroRNA Purification Kit • January 25, 2024
- Continued Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 30, 2024
- Extracting RNA and DNA from Fixed P. acuta Sections (newly fixed 2/8/24) • February 15, 2024
- Fixing Coral Tissues for LCM • April 29, 2024
- Fixing POC and POR Coral Tissues for LCM • July 3, 2024
- Further Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 24, 2024
- LCM Low Input RNA Library Prep • September 18, 2024
- LCM Pico Methyl-Seq Library Prep • September 17, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 1 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 2 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 3 • December 15, 2024
- LCM Test 3, New Slide Prep Protocol • June 5, 2024
- LCM Test 4, Porites and New Kits • July 18, 2024
- LCM Zymo Trio WGBS Library Prep • December 3, 2024
- Qiagen AllPrep DNA/RNA FFPE Kit Protocol • January 25, 2024
- Suucessful? Testing of Charm Biotech DNA Extraction on LCM-d P. acuta • June 26, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned P. comp and M. cap • February 9, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • June 17, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • May 14, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned P. comp and M. cap • February 1, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • May 13, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Testing NEBNext Low Input RNA Library Prep • August 1, 2024
- Testing Pico Methyl-Seq Library Prep • August 27, 2024
- Testing Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 19, 2024
- Testing Zymo Quick RNA Microprep Kit on Cryosectioned and LCM-d P. acuta • June 13, 2024
- Testing Zymo cfDNA DNA Extraction on Cryosectioned and LCM-d P. acuta • May 28, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa • July 19, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa and P. acuta • July 24, 2024
LCM Pilot 3
- 2024-08-28 LCM Experiment Sample Prep • August 28, 2024
- 2024-09-08 LCM Experiment Test Extraction • September 8, 2024
- 2024-09-13 LCM Experiment Extractions • September 13, 2024
Library Prep 8
- LCM Low Input RNA Library Prep • September 18, 2024
- LCM Pico Methyl-Seq Library Prep • September 17, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 1 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 2 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 3 • December 15, 2024
- LCM Zymo Trio WGBS Library Prep • December 3, 2024
- Testing NEBNext Low Input RNA Library Prep • August 1, 2024
- Testing Pico Methyl-Seq Library Prep • August 27, 2024
Madracis decactis 15
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
- 2023-05-08 ENCORE DLAB, MCAV, and MDEC TagSeq (RNA) Plate Prep • May 8, 2023
- 2023-05-09 Project Summary, 2022-2023 RNA and DNA Extractions for ENCORE MDEC Project • May 9, 2023
Montastraea cavernosa 14
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
- 2023-05-08 ENCORE DLAB, MCAV, and MDEC TagSeq (RNA) Plate Prep • May 8, 2023
Montipora 2
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Sending FF and PX corals for snSeq Trials • January 29, 2024
OysterPaper 2
- Point Judith Oyster RNAseq QC • January 12, 2023
- Point Judith Oyster RNAseq Analysis • February 27, 2023
PAXgene 19
- 2023-03-21 Paxgene Fixation, Stabilization, and Extraction Test • March 21, 2023
- 2023-04-13 Paxgene Fixation, Stabilization, and Extraction Test • April 13, 2023
- 2024-08-28 LCM Experiment Sample Prep • August 28, 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Genomic DNA Purification Kit • January 31, 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Total RNA/MicroRNA Purification Kit • January 25, 2024
- Extracting RNA and DNA from Fixed P. acuta Sections (newly fixed 2/8/24) • February 15, 2024
- Fixing Coral Tissues for LCM • April 29, 2024
- Fixing Coral Tissues for Protocol Testing • August 30, 2023
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Fixing POC and POR Coral Tissues for LCM • July 3, 2024
- Protocol for Cryoembedding Tissues in OCT Media for Cryosectioning (for FISH, Immunofluorescence, and LCM) • August 22, 2023
- Protocol for Cryosectioning Tissues (for FISH, Immunofluorescence, and LCM) • August 31, 2023
- Protocol for Fixing Coral Tissues with PAXgene Tissue Fixative followed by EDTA Decalcification • August 21, 2023
- Qiagen AllPrep DNA/RNA FFPE Kit Protocol • January 25, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned P. comp and M. cap • February 9, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned P. comp and M. cap • February 1, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
Physiology 6
- Putnam Lab Physiology Airbrushing Protocol • June 19, 2023
- Putnam Lab Physiology Ash Free Dry Weight Protocol • June 23, 2023
- Putnam Lab Physiology Chlorophyll Protocol • June 19, 2023
- Putnam Lab Physiology Soluble Protein Protocol • June 22, 2023
- Putnam Lab Physiology Surface Area by Wax Dipping • June 30, 2023
- Putnam Lab Physiology Symbiont Density Protocol • June 19, 2023
Pocillopora 43
- 2023-03-15 E5 Site 1 Species ID (POC and POR) via PCR and Sanger Sequencing • March 15, 2023
- 2023-03-21 Paxgene Fixation, Stabilization, and Extraction Test • March 21, 2023
- 2023-04-11 E5 Sites 2 & 3 Species ID (POC and POR) via PCR and Sanger Sequencing • April 11, 2023
- 2023-04-11 Heron Island Pdam Species ID (mtORF confirmation) • April 11, 2023
- 2023-04-13 Paxgene Fixation, Stabilization, and Extraction Test • April 13, 2023
- 2024-08-28 LCM Experiment Sample Prep • August 28, 2024
- 2024-09-08 LCM Experiment Test Extraction • September 8, 2024
- 2024-09-13 LCM Experiment Extractions • September 13, 2024
- Continued Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 30, 2024
- Extracting RNA and DNA from Fixed P. acuta Sections (newly fixed 2/8/24) • February 15, 2024
- Fixing Coral Tissues for LCM • April 29, 2024
- Fixing Coral Tissues for Protocol Testing • August 30, 2023
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Fixing POC and POR Coral Tissues for LCM • July 3, 2024
- Further Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 24, 2024
- LCM Low Input RNA Library Prep • September 18, 2024
- LCM Pico Methyl-Seq Library Prep • September 17, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 1 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 2 • December 6, 2024
- LCM Pilot Zymo Trio WGBS Library Prep Round 3 • December 15, 2024
- LCM Test 3, New Slide Prep Protocol • June 5, 2024
- LCM Test 4, Porites and New Kits • July 18, 2024
- LCM Zymo Trio WGBS Library Prep • December 3, 2024
- PCR Protocol • March 14, 2023
- Protocol for Cryoembedding Tissues in OCT Media for Cryosectioning (for FISH, Immunofluorescence, and LCM) • August 22, 2023
- Protocol for Cryosectioning Tissues (for FISH, Immunofluorescence, and LCM) • August 31, 2023
- Protocol for Fixing Coral Tissues with PAXgene Tissue Fixative followed by EDTA Decalcification • August 21, 2023
- Sending FF and PX corals for snSeq Trials • January 29, 2024
- Suucessful? Testing of Charm Biotech DNA Extraction on LCM-d P. acuta • June 26, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned P. comp and M. cap • February 9, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • June 17, 2024
- Testing Charm Biotech DNA Extraction on Cryosectioned and LCM-d P. acuta • May 14, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned P. comp and M. cap • February 1, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • May 13, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Testing NEBNext Low Input RNA Library Prep • August 1, 2024
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
- Testing Pico Methyl-Seq Library Prep • August 27, 2024
- Testing Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 19, 2024
- Testing Zymo Quick RNA Microprep Kit on Cryosectioned and LCM-d P. acuta • June 13, 2024
- Testing Zymo cfDNA DNA Extraction on Cryosectioned and LCM-d P. acuta • May 28, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa and P. acuta • July 24, 2024
Pocillopora damicornis 9
- 2022-10-27 Barott and Brown Pdam RNA and DNA Extractions • October 27, 2022
- 2022-11-02 Barott and Brown Pdam RNA and DNA Extractions • November 2, 2022
- 2022-11-08 Barott and Brown Pdam RNA and DNA Extractions • November 8, 2022
- 2022-11-11 Barott and Brown Pdam RNA and DNA Extractions • November 11, 2022
- 2022-11-18 Barott and Brown Pdam RNA and DNA Extractions • November 18, 2022
- 2022-12-02 Barott and Brown Pdam RNA Nanodrop • December 2, 2022
- 2022-12-02 Barott and Brown Pdam RNA Plate Prep • December 2, 2022
- 2023-01-27 Project Summary Barott and Brown Pdam RNA and DNA Extractions • January 27, 2023
- 2023-04-11 Heron Island Pdam Species ID (mtORF confirmation) • April 11, 2023
Porites 14
- 2023-03-15 E5 Site 1 Species ID (POC and POR) via PCR and Sanger Sequencing • March 15, 2023
- 2023-04-11 E5 Sites 2 & 3 Species ID (POC and POR) via PCR and Sanger Sequencing • April 11, 2023
- Continued Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 30, 2024
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Fixing POC and POR Coral Tissues for LCM • July 3, 2024
- Further Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 24, 2024
- LCM Test 4, Porites and New Kits • July 18, 2024
- PCR Protocol • March 14, 2023
- Sending FF and PX corals for snSeq Trials • January 29, 2024
- Testing NEBNext Low Input RNA Library Prep • August 1, 2024
- Testing Pico Methyl-Seq Library Prep • August 27, 2024
- Testing Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 19, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa • July 19, 2024
- Testing of Charm Biotech DNA Extraction on LCM-d P. compressa and P. acuta • July 24, 2024
Porites astreoides 18
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2022-10-19 ENCORE RNA and DNA Extractions • October 19, 2022
- 2022-10-21 ENCORE RNA and DNA Extractions • October 21, 2022
- 2022-10-24 ENCORE RNA and DNA Extractions • October 24, 2022
- 2022-10-26 ENCORE RNA and DNA Extractions • October 26, 2022
- 2022-10-31 ENCORE RNA and DNA Extractions • October 31, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
Protocol 20
- 2024-09-13 LCM Experiment Extractions • September 13, 2024
- Adult Coral RNA and DNA Extraction using the Zymo Quick-DNA/RNA™ Miniprep Plus Kit • October 3, 2022
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Genomic DNA Purification Kit • January 31, 2024
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Total RNA/MicroRNA Purification Kit • January 25, 2024
- PCR Protocol • March 14, 2023
- PPP Lab Agarose Gel Protocol • October 2, 2022
- PPP Lab RNA Tape Station Protocol • October 2, 2022
- Protocol for Cryoembedding Tissues in OCT Media for Cryosectioning (for FISH, Immunofluorescence, and LCM) • August 22, 2023
- Protocol for Cryosectioning Tissues (for FISH, Immunofluorescence, and LCM) • August 31, 2023
- Protocol for Fixing Coral Tissues with PAXgene Tissue Fixative followed by EDTA Decalcification • August 21, 2023
- Putnam Lab Physiology Airbrushing Protocol • June 19, 2023
- Putnam Lab Physiology Ash Free Dry Weight Protocol • June 23, 2023
- Putnam Lab Physiology Chlorophyll Protocol • June 19, 2023
- Putnam Lab Physiology Soluble Protein Protocol • June 22, 2023
- Putnam Lab Physiology Surface Area by Wax Dipping • June 30, 2023
- Putnam Lab Physiology Symbiont Density Protocol • June 19, 2023
- Qiagen AllPrep DNA/RNA FFPE Kit Protocol • January 25, 2024
- RNA Nanodrop Purity Check and Quantification Protocol • October 2, 2022
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
- dsDNA and RNA Qubit Quantification Protocol • October 2, 2022
RNA 58
- 2022-10-03 ENCORE RNA and DNA Extractions • October 3, 2022
- 2022-10-05 ENCORE RNA and DNA Extractions • October 5, 2022
- 2022-10-17 ENCORE RNA and DNA Extractions • October 17, 2022
- 2022-10-19 ENCORE RNA and DNA Extractions • October 19, 2022
- 2022-10-21 ENCORE RNA and DNA Extractions • October 21, 2022
- 2022-10-24 ENCORE RNA and DNA Extractions • October 24, 2022
- 2022-10-26 ENCORE RNA and DNA Extractions • October 26, 2022
- 2022-10-27 Barott and Brown Pdam RNA and DNA Extractions • October 27, 2022
- 2022-10-31 ENCORE RNA and DNA Extractions • October 31, 2022
- 2022-11-02 Barott and Brown Pdam RNA and DNA Extractions • November 2, 2022
- 2022-11-08 Barott and Brown Pdam RNA and DNA Extractions • November 8, 2022
- 2022-11-11 Barott and Brown Pdam RNA and DNA Extractions • November 11, 2022
- 2022-11-18 Barott and Brown Pdam RNA and DNA Extractions • November 18, 2022
- 2022-12-02 Barott and Brown Pdam RNA Nanodrop • December 2, 2022
- 2022-12-02 Barott and Brown Pdam RNA Plate Prep • December 2, 2022
- 2023-01-13 ENCORE RNA and DNA Extractions • January 13, 2023
- 2023-01-18 ENCORE RNA and DNA Extractions • January 18, 2023
- 2023-01-23 E5 ACR Deep Dive RNA and DNA Extractions • January 23, 2023
- 2023-01-27 Project Summary Barott and Brown Pdam RNA and DNA Extractions • January 27, 2023
- 2023-02-16 ENCORE RNA and DNA Extractions • February 16, 2023
- 2023-03-02 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • March 2, 2023
- 2023-03-21 Paxgene Fixation, Stabilization, and Extraction Test • March 21, 2023
- 2023-04-06 ENCORE RNA and DNA Extractions • April 6, 2023
- 2023-04-13 Paxgene Fixation, Stabilization, and Extraction Test • April 13, 2023
- 2023-04-18 ENCORE RNA and DNA Extractions • April 18, 2023
- 2023-04-21 ENCORE RNA and DNA Extractions • April 21, 2023
- 2023-04-25 ENCORE RNA and DNA Extractions • April 25, 2023
- 2023-04-27 ENCORE RNA and DNA Re-Extractions • April 27, 2023
- 2023-05-05 Pooling RNA Using Zymo Clean and Concentrate Kit for ENCORE Project • May 5, 2023
- 2023-05-08 ENCORE DLAB, MCAV, and MDEC TagSeq (RNA) Plate Prep • May 8, 2023
- 2023-05-09 Project Summary, 2022-2023 RNA and DNA Extractions for ENCORE MDEC Project • May 9, 2023
- 2024-08-01 E5 Time Series RNA and DNA Re-Extractions • August 1, 2024
- 2024-09-08 LCM Experiment Test Extraction • September 8, 2024
- 2024-09-13 LCM Experiment Extractions • September 13, 2024
- Adult Coral RNA and DNA Extraction using the Zymo Quick-DNA/RNA™ Miniprep Plus Kit • October 3, 2022
- Charm Biotech Just-a-Tube ™ Laser Captured Microdissection (LCM) Sample Total RNA/MicroRNA Purification Kit • January 25, 2024
- Continued Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 30, 2024
- Extracting RNA and DNA from Fixed P. acuta Sections (newly fixed 2/8/24) • February 15, 2024
- Fixing Coral Tissues for Protocol Testing • August 30, 2023
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
- Further Testing of Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 24, 2024
- Gene Expression Pipeline • March 12, 2024
- LCM Low Input RNA Library Prep • September 18, 2024
- PPP Lab RNA Tape Station Protocol • October 2, 2022
- Point Judith Oyster RNAseq QC • January 12, 2023
- Point Judith Oyster RNAseq Analysis • February 27, 2023
- Qiagen AllPrep DNA/RNA FFPE Kit Protocol • January 25, 2024
- RNA Nanodrop Purity Check and Quantification Protocol • October 2, 2022
- Testing Charm Biotech DNA Extraction on Cryosectioned P. comp and M. cap • February 9, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned P. comp and M. cap • February 1, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • May 13, 2024
- Testing Charm Biotech RNA Extraction on Cryosectioned and LCM-d P. acuta • January 25, 2024
- Testing Leica LDM7 Laser Capture Microdissection on P. acuta • October 16, 2023
- Testing NEBNext Low Input RNA Library Prep • August 1, 2024
- Testing PAXgene, PFA, (and Z-fix) for Immunofluorescence, FISH + LCM preps and sectioning • August 14, 2023
- Testing Zymo Quick DNA/RNA Microprep Kit on LCM-d P. acuta and P. compressa • July 19, 2024
- Testing Zymo Quick RNA Microprep Kit on Cryosectioned and LCM-d P. acuta • June 13, 2024
- dsDNA and RNA Qubit Quantification Protocol • October 2, 2022
Seriatopora 1
- Fixing Coral Tissues of Four Species to Compare Tissue Thickness • October 10, 2023
TagSeq 1
- Gene Expression Pipeline • March 12, 2024
snSeq 1
- Sending FF and PX corals for snSeq Trials • January 29, 2024