Prep and submit RNA for ENCORE Project, May 8, 2023 (to be sent to UT Austin sometime this summer)

Dilute extracted RNA to 12 ug/uL in 25 uL and consolidate into one plate for TagSeq. This plate prep guide is based off of Dr. Kevin Wong’s protocol/example here and my previous post here.

Samples

All DLAB, MDEC, and MCAV – 119 samples.

colony_id	RNA_QBIT_AVG	Amount to dilute, final concentration of 12 ng/uL in 25 uL	Amount of water	Volume in Tube currently	Amount left in tube after this	Plate Map Order	Plate Wells
Dlab-F1	15.9	19	6	40	21	1	Plate1_A1
Mdec-D4	12.7	24	1	90	66	2	Plate1_B1
Mcav-E1	27.3	11	14	50	39	3	Plate1_C1
Dlab-A2	17.4	17	8	35	18	4	Plate1_D1
Mdec-A5	23.4	13	12	90	77	5	Plate1_E1
Mcav-B5	18.1	17	8	24	7	6	Plate1_F1
Dlab-C5	13.4	22	3	40	18	7	Plate1_G1
Dlab-A1	12.6	24	1	90	66	8	Plate1_H1
Mdec-A7	18.3	16	9	50	34	9	Plate1_A2
Dlab-F2	18	17	8	35	18	10	Plate1_B2
Dlab-C7	17.15	17	8	24	7	11	Plate1_C2
Mcav-E2	27.4	11	14	90	79	12	Plate1_D2
Mdec-D5	14.3	21	4	40	19	13	Plate1_E2
Dlab-C6	13.5	22	3	40	18	14	Plate1_F2
Mcav-B6	17	18	7	24	6	15	Plate1_G2
Dlab-A3	13.55	22	3	24	2	16	Plate1_H2
Mcav-E3	19.9	15	10	90	75	17	Plate1_A3
Mcav-B7	23.3	13	12	90	77	18	Plate1_B3
Mdec-B1	13.3	23	2	90	67	19	Plate1_C3
Dlab-F3	19.1	16	9	24	8	20	Plate1_D3
Mdec-D6	15.8	19	6	24	5	21	Plate1_E3
Dlab-F4	18.9	16	9	24	8	22	Plate1_F3
Dlab-D1	12.3	24	1	50	26	23	Plate1_G3
Dlab-A4	13.5	22	3	50	28	24	Plate1_H3
Mdec-B3	14.4	21	4	90	69	25	Plate1_A4
Dlab-D2	21.2	14	11	40	26	26	Plate1_B4
Dlab-F5	14	21	4	24	3	27	Plate1_C4
Dlab-A5	15.65	19	6	24	5	28	Plate1_D4
Mcav-C1	18.5	16	9	90	74	29	Plate1_E4
Mdec-D7	37.6	8	17	90	82	30	Plate1_F4
Mcav-C2	14.9	20	5	90	70	31	Plate1_G4
Mcav-E4	12	25	0	90	65	32	Plate1_H4
Dlab-F6	25.5	12	13	24	12	33	Plate1_A5
Dlab-A6	12	25	0	40	15	34	Plate1_B5
Dlab-D3	12.2	25	0	90	65	35	Plate1_C5
Mcav-E5	17.45	17	8	24	7	36	Plate1_D5
Mdec-B4	15.3	20	5	90	70	37	Plate1_E5
Mdec-E1	22	14	11	50	36	38	Plate1_F5
Mcav-E6	17.25	17	8	24	7	39	Plate1_G5
Mcav-C3	21.6	14	11	90	76	40	Plate1_H5
Mcav-C4	12.2	25	0	90	65	41	Plate1_A6
Dlab-A7	17.55	17	8	24	7	42	Plate1_B6
Mdec-B5	16.9	18	7	50	32	43	Plate1_C6
Dlab-D4	12	25	0	40	15	44	Plate1_D6
Mcav-F1	34.4	9	16	90	81	45	Plate1_E6
Dlab-F7	18	17	8	40	23	46	Plate1_F6
Mdec-E2	17.5	17	8	90	73	47	Plate1_G6
Mdec-B6	18.9	16	9	90	74	48	Plate1_H6
Mdec-E3	21	14	11	90	76	49	Plate1_A7
Mcav-C5	14.8	20	5	23	3	50	Plate1_B7
Mcav-F2	24.1	12	13	90	78	51	Plate1_C7
Mcav-A1	13.3	23	2	90	67	52	Plate1_D7
Mdec-B7	14.9	20	5	90	70	53	Plate1_E7
Dlab-D5	14	21	4	40	19	54	Plate1_F7
Mdec-E5	28.2	11	14	90	79	55	Plate1_G7
Dlab-B1	12.35	24	1	24	0	56	Plate1_H7
Dlab-B2	19.4	15	10	40	25	57	Plate1_A8
Dlab-D6	13.4	22	3	24	2	58	Plate1_B8
Mdec-C1	26.3	11	14	90	79	59	Plate1_C8
Mcav-F3	12.1	25	0	90	65	60	Plate1_D8
Mdec-E6	22.1	14	11	90	76	61	Plate1_E8
Mcav-C6	14	21	4	40	19	62	Plate1_F8
Mcav-A2	17.2	17	8	90	73	63	Plate1_G8
Dlab-B3	18.5	16	9	35	19	64	Plate1_H8
Dlab-D7	19.6	15	10	40	25	65	Plate1_A9
Mdec-E7	26.1	11	14	90	79	66	Plate1_B9
Mdec-C2	33	9	16	90	81	67	Plate1_C9
Mcav-F4	18.2	16	9	40	24	68	Plate1_D9
Dlab-E1	16.2	19	6	40	21	69	Plate1_E9
Mcav-C7	17.1	18	7	90	72	70	Plate1_F9
Dlab-B4	12.5	24	1	40	16	71	Plate1_G9
Mcav-A3	17.2	17	8	90	73	72	Plate1_H9
Mcav-A4	24.1	12	13	90	78	73	Plate1_A10
Mdec-C3	12.4	24	1	90	66	74	Plate1_B10
Mcav-D1	14.5	21	4	90	69	75	Plate1_C10
Dlab-B5	16.05	19	6	24	5	76	Plate1_D10
Mcav-F5	18.55	16	9	24	8	77	Plate1_E10
Mdec-F1	53.7	6	19	90	84	78	Plate1_F10
Mcav-A5	17.1	18	7	24	6	79	Plate1_G10
Dlab-E2	25.1	12	13	40	28	80	Plate1_H10
Mcav-F6	15.3	20	5	40	20	81	Plate1_A11
Mcav-D2	15.9	19	6	90	71	82	Plate1_B11
Mdec-C4	25.6	12	13	90	78	83	Plate1_C11
Mcav-D3	19.4	15	10	90	75	84	Plate1_D11
Dlab-B6	15.4	19	6	40	21	85	Plate1_E11
Mdec-F2	28.1	11	14	90	79	86	Plate1_F11
Dlab-E3	15	20	5	40	20	87	Plate1_G11
Mcav-A6	15.1	20	5	90	70	88	Plate1_H11
Mcav-A7	23.5	13	12	50	37	89	Plate1_A12
Dlab-E4	14.1	21	4	24	3	90	Plate1_B12
Mcav-D4	16.9	18	7	90	72	91	Plate1_C12
Mdec-F3	15.8	19	6	90	71	92	Plate1_D12
Mdec-A1	13.3	23	2	24	1	93	Plate1_E12
Mcav-F7	15.3	20	5	24	4	94	Plate1_F12
Dlab-B7	13.7	22	3	40	18	95	Plate1_G12
Mdec-C6	16.7	18	7	90	72	96	Plate1_H12
Mcav-B1	14.8	20	5	90	70	97	Plate2_A1
Mdec-F4	22.1	14	11	50	36	98	Plate2_B1
Mdec-A2	22.8	13	12	90	77	99	Plate2_C1
Mcav-D5	16.4	18	7	50	32	100	Plate2_D1
Dlab-C2	17.3	17	8	90	73	101	Plate2_E1
Mdec-D1	27.3	11	14	90	79	102	Plate2_F1
Dlab-E5	14.4	21	4	40	19	103	Plate2_G1
Mdec-C7	21.1	14	11	90	76	104	Plate2_H1
Dlab-E6	12.25	23	2	23	0	105	Plate2_A2
Dlab-C3	14	21	4	40	19	106	Plate2_B2
Mcav-B2	27.1	11	14	90	79	107	Plate2_C2
Mdec-F5	15.1	20	5	90	70	108	Plate2_D2
Mdec-D2	21.7	14	11	90	76	109	Plate2_E2
Mcav-D6	19	16	9	24	8	110	Plate2_F2
Mdec-A3	15.7	19	6	90	71	111	Plate2_G2
Mdec-F6	14.5	21	4	90	69	112	Plate2_H2
Mdec-A4	15.85	19	6	24	5	113	Plate2_A3
Dlab-E7	17.8	17	8	90	73	114	Plate2_B3
Mdec-D3	31	10	15	90	80	115	Plate2_C3
Mcav-B4	17.1	18	7	50	32	116	Plate2_D3
Mcav-D7	16	19	6	90	71	117	Plate2_E3
Dlab-C4	13	23	2	35	12	118	Plate2_F3
Mdec-F7	21.5	14	11	90	76	119	Plate2_G3

Prepped plates were covered with foil and placed in -80 until shipping: